Please attend your poster at the designated poster session (odd poster numbers on June 20th, even numbers – on June 21st). We suggest to have the posters up until the end of the conference.
Space available for your poster will be 95 cm (wide) x 150 cm (height). Recommended poster size is A0 format (84 cm x 118.8 cm, portrait orientation).
|
POSTER SESSION I |
POSTER SESSION II |
| [P.001] Structure and Function of Cas6 from S. thermophilus Type III-A system Giedrė Tamulaitienė*, Irmantas Mogila, Miglė Kazlauskienė, Gintautas Tamulaitis, Virginijus Šikšnys Institute of Biotechnology, Vilnius University, Lithuania |
[P.002] In vitro characterization of diverse Cas12a nucleases Megumu Mabuchi*, Ryan T. Fuchs, Audrey Noireterre, Jennifer L. Curcuru, Hannah Blakely, Joseph M. Whipple, Ezra Schildkraut, G. Brett Robb New England BioLabs Inc. USA |
| [P.003] Identification of novel anti-CRISPR proteins in self-targeting genomes Kyle E. Watters*, Jennifer A. Doudna Department of Molecular and Cell Biology, University of California Berkeley, United States |
[P.004] Conformational dynamics of the Cas12a interference complex Joshua C. Cofsky* (1), Janice Chen (1), Lucas Harrington (1), Enbo Ma (1), John Kuriyan (1-5), Jennifer A. Doudna (1-3,5-6) (1) Department of Molecular and Cell Biology, University of California, Berkeley, USA, (2) Howard Hughes Medical Institute, University of California, Berkeley, USA, (3) Department of Chemistry, University of California, Berkeley, USA, (4) California Institute for Quantitative Biosciences, University of California, Berkeley, USA, (5) Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, USA, (6) Innovative Genomics Institute, University of California, Berkeley, USA |
| [P.005] Cis-acting determinants for spacer DNA uptake into Pyrococcus furiosus CRISPR arrays Elizabeth A. Watts* (1), Julie Grainy (2), Sandra Garrett (4), Landon Clark (1), Brenton Graveley (4), Michael P. Terns (1,2,3) (1) Department of Biochemistry and Molecular Biology, University of Georgia, USA, (2) Department of Microbiology, University of Georgia, USA, (3) Department of Genetics, University of Georgia, USA, (4) Department of Genetics and Genome Sciences, UConn Health, USA |
[P.006] A bibliographic analysis of patents on CRISPR Byoung Min Ahn*, Min-Kyu Han Korea Institute of S&T Evaluation and Planning (KISTEP), South Korea |
| [P.007] Multiple families of anti-CRISPR proteins encoded by a single archaeal virus SIRV2 Yuvaraj Bhoobalan-Chitty, Fei He, Xu Peng* Danish Archaea Center, Department of Biology, University of Copenhagen, Denmark |
[P.008] Characterizing a thermostable Cas9 for bacterial genome editing and silencing Prarthana Mohanraju* (1), Ioannis Mougiakos (1), Elleke F. Bosma (1,2), Valentijn Vrouwe (1), Max Finger Bou (1), Mihris I. S. Naduthodi (1), Alex Gussak (1), Rudolf B. L. Brinkman (3), Richard van Kranenburg (1,3), John van der Oost (1) (1) Laboratory of Microbiology, Wageningen University, The Netherlands, (2) Corbion, The Netherlands, (3) The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark |
| [P.009] Deletion of recD indirectly reduce adaptation in type I-E CRISPR-Cas system Lina Amlinger, Martin Larsson, Sanna Koskiniemi, Magnus Lundgren* Dept. of Cell and Molecular Biology, Uppsala University, Sweden |
[P.010] Mechanistic similarities and differences in destabilization of duplex DNA by Cas9, Cas12a and Cas12b CRISPR-Cas nucleases Vladimir Mekler* (1), Leonid Minakhin (1), Konstantin Kuznedelov (1), Karthik Murugan (2), Dipali G. Sashital (2), Kontantin Severinov (1,3,4) (1) Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, USA, (2) Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Ames, IA, USA, (3) Institutes of Molecular Genetics and Gene Biology, Russian Academy of Sciences, Moscow, Russia, (4)Peter the Great State Polytechnical Institute, St. Petersburg, Russia |
| [P.011] Adaptation in CRISPR Type V-A Wen Y. Wu* (1), Meral Türen (1), Alexander Bartels (1), Stan J.J. Brouns (2), Raymond H. J. Staals (1), John van der Oost (1) (1) Wageningen University and Research, The Netherlands, (2) Delft University of Technology, The Netherlands |
[P.012] Cas9 of Campylobacter jejuni induces DNA damage in the presence of metal ions Chinmoy Saha* (1), Prarthana Mohanraju (2), Andrew Stubbs (3), Gaurav Dugar (4), Youri Hoogstrate (3), Gert-Jan Kremers (5), Wiggert A. van Cappellen (5), Deborah Horst-Kreft (1), Adriaan B. Houtsmuller (5), Serena Bruens (6), Duncan Gaskin (7), Dior Beerens (1), Maarten Klunder (2), Jeroen A. A. Demmers (8), Dik van Gent (6), Johan W. Mouton (1), David S. Weiss (9), Peter J. van der Spek (3), Peter van Baarlen (10), John van der Oost (2), Rogier Louwen (1) (1) Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre, Rotterdam, The Netherlands, (2) Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands, (3) Department of Bioinformatics, Erasmus University Medical Center, Rotterdam, The Netherlands, (4) Institute of Molecular Infection Biology (IMIB)/ Research Center for Infectious Diseases (ZINF), University of Würzburg, Würzburg, Germany, (5) Optical Imaging Center, Erasmus University Medical Center, Rotterdam, The Netherlands, (6) Department of Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands, (7) Institute of Food Research, Gut Health and Food Safety Programme, Norwich Research Park, Norwich, United Kingdom, (8) Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands, (9) Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA, (10) Host–Microbe Interactomics Group, Wageningen University, Wageningen, The Netherlands |
| [P.013] CRISPR RNA processing by a Type IV Cas6 endoribonuclease Hannah N. Taylor*, Emily Warner, Valerie Crowley, Ryan N. Jackson Utah State University, Department of Chemistry and Biochemistry, USA |
[P.014] Targeted reporter gene knock-in to generate human induced pluripotent stem cell line for high-throughput screening via CRISPR-Cas9 Ji-Woo Kim*, Young-Jun Choi, Hyemin Kim, Han-Jin Park Predictive Toxicology department, Korea Institute of Toxicology |
| [P.015] Acquisition of new spacers by Type III CRISPR array in a natural T. thermophilus strain Daria Artamonova* (1,2), Anna Lopatina (2), Ekaterina Semenova (3), Konstantin Severinov (1,2,3) (1) Skolkovo Institute of Science and Technology, Skolkovo, Russia, (2) Institute of Gene Biology RAS, Institution, Moscow, Russia, (3) Waksman Institute of Microbiology, Rutgers University, NJ, USA |
[P.016] Accurate analysis of genuine CRISPR editing events with ampliCan Kornel Labun* (1), Xiaoge Guo (2,3), Alejandro Chavez (4), George Church (2,3), James Gagnon (5), Eivind Valen (1,6) (1) Department of Informatics/Computational Biology Unit; University of Bergen; Bergen, 5008; Norway, (2) Wyss Institute for Biologically Inspired Engineering; Harvard University; Cambridge, MA 02115; USA, (3) Department of Genetics; Harvard Medical School; Boston, Massachusetts, 02115; USA, (4) Department of Pathology and Cell Biology; Columbia University; New York, NY 10032; USA, (5) Department of Biology; University of Utah; Salt Lake City, UT 84112; USA, (6) Sars International Centre for Marine Molecular Biology; University of Bergen; Bergen, 5008; Norway |
| [P.017] The activity of type I-E, I-F and III-A CRISPR-Cas systems during phage infection Lucia M. Malone* (1), Carolin Warnecke (1), Torsten Kleffmann (2), Xochitl C. Morgan (1), Peter C. Fineran (1) (1) Department of Immunology and Microbiology, University of Otago, New Zealand, (2) Department of Biochemistry, University of Otago, New Zealand |
[P.018] CRISPR/Cas9 searches for a target by lateral diffusion Viktorija Globyte* (1), Seung Hwan Lee (1,2), Taegun Bae (2), Jin-soo Kim (2,3), Chirlmin Joo (1) (1) Kavli Institute of Nanoscience and Department of BioNanoScience, Delft University of Technology, Delft, The Netherlands, (2) Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea, (3) Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea |
| [P.019] Sequence determinants that influence spacer adaptation efficiency D.Vygovskyi* (1), B.Kirillov (2), O.Musharova (2,3), E.Savitskaya (2,3) (1) Moscow Institute of Physics and Technology, Moscow, Russia, (2) Institute of Science and Technology, Skolkovo, Russia, (3) Institutes of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia |
[P.020] A rapid method to interrogate the protospacer adjacent motif biodiversity presented by Class2 CRISPR proteins Tautvydas Karvelis* (1), Giedrius Gasiunas* (2), Joshua K. Young (3), Greta Bigelyte (1), Tomas Urbaitis (2), Arunas Silanskas (1), Haining Lin (3), Matthew G. King (3), Mark Cigan (3,4), Virginijus Siksnys (1) (1) Institute of Biotechnology, Vilnius University, Lithuania, (2) CasZyme, Lithuania, (3) Corteva (Pioneer Hi-bred, Int.), USA, (4) Current address: Genus Research, Genus plc, USA |
| [P.021] In vivo detection of primed adaptation intermediates in Escherichia coli Anna Shiriaeva* (1,2,5), Ekaterina Savitskaya (1,3), Kirill Datsenko (4), Irina Vvedenskaya (5), Bryce Nickels (5), Ekaterina Semenova (5), Konstantin Severinov (1,2,3,5) (1) Skolkovo Institute of Science and Technology, Moscow, Russia, (2) Peter the Great St.Petersburg Polytechnic University, Saint-Petersburg, Russia, (3) Institute of Molecular Genetics of the Russian Academy of Sciences; Moscow, Russia, (4) Department of Biological Sciences, Purdue University, West Lafayette, USA, (5) Waksman Institute of Microbiology, The State University of New Jersey, Piscataway, NJ USA |
[P.022] Automated annotation of genomic CRISPR-Cas systems: predicting the boundaries of CRISPR elements Omer S. Alkhnbashi* (1), Shiraz A. Shah (2), Fabrizio Costa (3), Roger A. Garrett (2), Rolf Backofen (1) (1) Freiburg Bioinformatics Group, Department of Computer Science, University of Freiburg, Freiburg, Germany, (2) Danish Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark, (3) Department of Computer Science, College of Engineering, University of Exeter, Exeter, UK |
| [P.023] Pervasive acquisition of CRISPR memory from multiple replicons driven by inter-species mating of archaea Israela Turgeman-Grott* (1), Shirley Joseph (1), Sam Marton (1), Kim Eizenshtein (1), Adit Naor (1,2), Shannon Soucy (3), Aris-Edda Stachler (4), Yarden Shalev (1), Mor Zarkor (1), Leah Reshef (1), Neta Altman-Price (1), Anita Marchfelder (4), Uri Gophna (1) (1) Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel, (2) Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, (3) Department of Biological Sciences, Dartmouth College, 78 College Street, Hanover, New Hampshire, 03766, USA, (4) Department of Biology II, Ulm University, 89069 Ulm, Germany |
[P.024] Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair Natasa Savic*† (1), Femke C.A.S. Ringnalda† (1), Helen Lindsay (2), Christian Berk (3), Katja Bargsten (4), Yizhou Li (3), Dario Neri (3), Mark D. Robinson (2), Constance Ciaudo (1), Jonathan Hall (3), Martin Jinek (4), Gerald Schwank (1) (1) The Institute of Molecular Health Sciences, ETH Zurich, Otto-Stern-Weg 7, HPL, 8093 Zurich, Switzerland; (2) The Institute of Molecular Life Sciences and SIB Swiss Institute of Bioinformatics, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland; (3) Department of Chemistry and Applied Biosciences, Institute for Pharmaceutical Sciences, Vladimir-Prelog-Weg 1-5/10, 8093 Zurich, Switzerland; (4)Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland; † These authors contributed equally |
| [P.025] Towards a comprehensive census of CRISPR-linked genes Sergey A. Shmakov* (1,2), Kira S. Makarova (2), Yuri I. Wolf (2), Konstantin V. Severinov (1,3,4), Eugene V. Koonin (2) (1) Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia, (2) National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA, (3) Waksman Institute for Microbiology Rutgers, The State University of New Jersey Piscataway, NJ 08854, USA, (4) Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia |
[P.026] A growth-based PAM screen reveals non-canonical PAMs for the S. pyogenes Cas9 Daphne Collias* (1), Ryan Leenay (1), Rebecca Slotkowski (1), Chase L. Beisel (1,2) (1) North Carolina State University, Raleigh, NC USA, (2) Helmholtz Institute for RNA-based Infection Research, Würzburg Germany |
| [P.027] Evolution of immunity and counter-immunity in a Type I-C CRISPR-Cas system Lina M. Leon*, Joseph Bondy-Denomy Department of Microbiology & Immunology, University of California-San Francisco, USA |
[P.028] ICE: An Analysis Tool for CRISPR Editing Kevin Holden*, Tim Hsiau, Travis Maures, Kelsey Waite, Joyce Yang, Reed Kelso, Rich Stoner Synthego, USA |
| [P.029] RNA processing and activation of type IIIA CRISPR-Cas systems Brighton Samatanga* (1), Irmantas Mogila (2), Gintautas Tamulaitis (2), Virginijus Siksnys (2), Ralf Seidel (1) (1) Peter Debye Institute for Soft Matter Physics, University of Leipzig, Germany, (2) Department of Protein-DNA Interactions, Vilnius University, Lithuania |
[P.030] A CRISPR-Cas9 platform for genetic perturbations of brain extracellular matrix regulators in vivo Sverre Grødem* (1), Guro Sandvik (1), Jennifer Hazen (1), Kristian Lensjø (1), Torkel Hafting (2), Marianne Fyhn (1) (1) Department of Biosciences, University of Oslo, Norway, (2) Institute of Basic Medical Sciences, University of Oslo, Norway |
| [P.031] Avoidance of protospacer-associated motive in spacers acquired during CRISPR adaptation Ekaterina Savitskaya* (1,2), Daniil Vygovskyi (3), Olga Musharova (1,2), Sofia Medvedeva (1), Konstantin Severinov (1,2) (1) Skolkovo Institute of Science and Technology, Russia, (2) Institute of Molecular Genetics Russian Academy of Science, Russia, (3) Moscow Institute of Physics and Technology, Russia |
[P.032] Potato genome editing directed by microparticles functionalised with CRISPR/Cas9 RNP complexes Natalia Kalinina* (1,2), Andrey Khromov (1,2), Antonida Makhotenko (1,2), Ekaterina Snigir (1), Svetlana Makarova (1,2), Valentin Makarov (1,2), Tatyana Suprunova (1), Michael Taliansky (1,3) (1) Doka-Gene Technology Ltd, Rogachevo, Moscow Region, Russia, (2) Lomonosov Moscow State University, Moscow, Russia, (3) The James Hutton Institute, Dundee, United Kingdom |
| [P.033] A non-canonical seed sequence regulates targeting by the CRISPR/Cmr complex Yifan Zhu, David W. Taylor, Eline Stroobach, Sanne Klompe, Nirajan Neupane, Anna Sobieraj, Willem M. de Vos, Stan JJ Brouns, Akeo Shinkai, Jennifer A. Doudna, Raymond H.J. Staals*, John van der Oost Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, 6703 HB Wageningen, The Netherlands |
[P.034] Kinetic comparison of Cas9 homologs recognizing diverse PAM sequences Paul Yourik*, Ryan T. Fuchs, Megumu Mabuchi, Jennifer L. Curcuru, G. Brett Robb RNA and Genome Editing, New England Biolabs Inc., USA |
| [P.035] Cas4 facilitates PAM-compatible spacer selection during CRISPR adaptation Cristóbal Almendros*† (1), Sebastian N. Kieper† (1), Juliane Behler (2), Rebecca E. McKenzie (1), Franklin L. Nobrega (1), Anna C. Haagsma (1), Jochem N. A. Vink (1), Wolfgang R. Hess (2,3), Stan J.J. Brouns (1,4) (1) Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Delft, Netherlands, (2) Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Freiburg, Germany, (3) Freiburg Institute for Advanced Studies, University of Freiburg, Freiburg, Germany, (4) Laboratory of Microbiology, Wageningen University, Wageningen, Netherlands; † These authors contributed equally |
[P.036] Stable mitoCas9-BE4-Gam and mitoCas9-ABE7.10 lines as background for mtDNA editing systems Nikita Shebanov, Natalya Verechshagina*, Natalya Nikitchina, Ilia Mazunin Laboratory of Molecular Genetics Technologies, Immanuel Kant Baltic Federal University, Russia |
| [P.037] Influence of spacer length for DNA interference in a type I-F CRISPR-Cas system Tomas Sinkunas*, Marija Fiodorovaitė, Inga Songailienė, Giedrė Tamulaitienė, Virginijus Siksnys Vilnius University Institute of Biotechnology, Department of Protein – DNA Interactions, Sauletekis al. 7, LT-10257, Vilnius, Lithuania |
[P.038] Conservation of non-specific single-stranded DNA degradation by Type V CRISPR systems Lucas B. Harrington* (1), Janice S. Chen (1), Enbo Ma (1), Joshua C. Cofsky (1), Jennifer Doudna (1-6) (1) Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA, (2) Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA, (3) Department of Chemistry, University of California, Berkeley, CA 94720, USA, (4) Innovative Genomics Institute, University of California, Berkeley, CA 94704, USA, (5) Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA, (6) Molecular Biophysics, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA |
| [P.039] Discovery and characterization of a novel two-domain Class I anti-CRISPR protein Chantel N. Trost* (1), Eric Lu (2), Marios Mejdani (2), Bianca Garcia (1), Karen L. Maxwell (2), Alan R. Davidson (1,2) (1) Department of Molecular Genetics, University of Toronto, Canada, (2) Department of Biochemistry, University of Toronto, Canada |
[P.040] CRISPR-Cas Systems in Multicellular Cyanobacteria Wolfgang R. Hess* (1), Shengwei Hou (1), Manuel Brenes-Álvarez (2), Viktoria Reimann (1), Omer S. Alkhnbashi (3), Rolf Backofen (3), Alicia M. Muro-Pastor (2) (1) Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Germany, (2) Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Seville, Spain, (3) Bioinformatics group, Department of Computer Science, University of Freiburg, Germany |
| [P.041] Exploring type II-A spacer distribution Naama Aviram*, Luciano A. Marraffini Laboratory of Bacteriology, The Rockefeller University, New York, NY, USA |
[P.042] Generation of PXR (NR1I2) reporter human induced pluripotent stem cells Hyemin Kim*, Ji-Woo Kim, Han-Jin Park Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea |
| [P.043] Conformational Dynamics of DNA Binding and Cas3 Recruitment by the CRISPR RNA-guided Cascade Complex Paul B.G. van Erp* (1), Angela Patterson (2), Ravi Kant (2), Luke Berry (2), Sarah M. Golden (1), Brittney L. Forsman (1), Joshua J. Carter (1), Ryan N. Jackson (3), Brian Bothner (2), Blake Wiedenheft (1) (1) Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA, (2) Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA, (3) Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322, USA |
[P.044] Modification of RGEN/AsCpf1 elements for import and function inside of the human mitochondrion Natalya Nikitchina* (1), Natalya Verechshagina (1), Nikita Shebanov (1), Yuri Vyatkin (1,2), Konstantin Orishchenko (1,3), Ilia Mazunin (1) (1) Laboratory of Molecular Genetics Technologies, Immanuel Kant Baltic Federal University, Russia, (2) AcademGene LLC, Russia, (3) Laboratory of Cell Technologies, Institute of Cytology and Genetics SB RAS, Russia |
| [P.045] High Tolerance to Self-targeting in a halophilic Archaeon Lisa-Katharina Maier*† (1), Aris-Edda Stachler† (1), Israela Turgeman-Grott (2), Ella S. Segal (2), Thorsten Allers (3), Anita Marchfelder (1) and Uri Gophna (2) (1) Department of Biology II, Ulm University, 89069, Ulm, Germany, (2) Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel, (3) School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; † These authors contributed equally |
[P.046] Combining Barcode Libraries with Targeted Gene Expression for Single-Cell Genetic Analysis Alex Chenchik, Mikhail Makhanov, Paul Diehl*, Costa Frangou Cellecta, Inc., Mountain View, CA, USA |
| [P.047] RNA-Casing: Modulation of a minimal Type I CRISPR-Cas system for specific RNA wrapping and purification Daniel Gleditzsch* (1), Patrick Pausch (2,3), Hanna Müller-Esparza (1), Julia Wiegel (1), Thomas Heimerl (2,3), Florian Altegoer (2,3), Gert Bange (2,3), Lennart Randau (1,2) (1) Max Planck Institute for Terrestrial Microbiology, Marburg, Germany, (2) LOEWE Center for Synthetic Microbiology (Synmikro), (3) Philipps-University Marburg, Germany |
[P.048] In vivo CRISPR-Cas gene editing with no detectable genome-wide off-target mutations Pinar Akcakaya* (1), Maggie L. Bobbin (2,3), Jimmy A. Guo (2), Jose Malagon-Lopez (2,3), Kendell Clement (2,3), Sara P. Garcia (2), Mick D. Fellows (4), Michelle J. Porritt (1), Mike A. Firth (5), Alba Carreras (1), Tania Baccega (1), Frank Seeliger (6), Mikael Bjursell (1), Shengdar Q. Tsai (2,3,7), Nhu T. Nguyen (2), Roberto Nitsch (4), Lorenz M. Mayr (1,8), Luca Pinello (2,3), Mohammad Bohlooly-Y (1), Martin J. Aryee (2,3), Marcello Maresca (1), J. Keith Joung (2,3) (1) Discovery Biology, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden, (2) Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA, (3) Department of Pathology, Harvard Medical School, Boston, MA, USA, (4) New Drug Modalities, Drug Safety and Metabolism, IMED Biotech Unit, AstraZeneca, Cambridge, United Kingdom, (5) Quantitative Biology, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden, (6) Pathology Science, Drug Safety and Metabolism, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden, (7) Current address: Department of Hematology, St. Jude Children’s Research Hospital, Memphis, TN, USA, (8) Current address: GE Healthcare Life Sciences, The Grove Centre, White Lion Road, Amersham, United Kingdom |
| [P.049] Quorum sensing regulates both innate and adaptive bacterial defence systems Hannah G. Hampton*, Simon A. Jackson, Peter C. Fineran Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand |
[P.050] An active CRISPR-Cas system in a prevalent gut bacterium targeting foreign and domestic threats Paola Soto-Pérez* (1), Jordan E. Bisanz (1), Kathy N. Lam (1), Joel Berry (1), Joseph Bondy-Denomy (1), Peter J. Turnbaugh (1,2) (1) Department of Microbiology & Immunology, University of California, San Francisco, USA, (2) Chan Zuckerberg Biohub, San Francisco, USA |
| [P.051] Discovery of novel anti-CRISPR proteins Nicole D. Marino*, Jenny Zhang, Adair L. Borges, Joseph Bondy-Denomy Department of Microbiology and Immunology, University of California, San Francisco, CA, United States |
[P.052] The design of Chelidonium majus L. MLP/GRP knock-out mutants using CRISPR/Cas9 gene editing Robert Nawrot*, Oskar Musidlak Adam Mickiewicz University in Poznan, Faculty of Biology, Institute of Experimental Biology, Department of Molecular Virology, Poznan, Poland |
| [P.053] The role of non-catalytic Csm subunits in Streptococcus thermophilus type III-A CRISPR-Cas immunity Irmantas Mogila* (1), Migle Kazlauskiene (1), Skaiste Valinskyte (1,2), Giedre Tamulaitiene (1), Gintautas Tamulaitis* (1), Virginijus Siksnys (1) (1) Institute of Biotechnology, Vilnius University, Saulėtekio av. 7, 10257 Vilnius, Lithuania, (2) Present address: Thermo Fisher Scientific Baltics, Graičiūno 8, 02241 Vilnius, Lithuania |
[P.054] Decoding the Mechanism of RNA-Targeting by Cas13a CRISPR-Cas System Ishita Jain* (1,2), Ekaterina Semenova (1), Konstantin Severinov (1,2) (1) Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, USA, (2) Skolkovo Institute of Science and Technology, Skolkovo, Russia |
| [P.055] Slipped spacers establish immune diversity via primed CRISPR adaptation Simon A. Jackson* (1), Nils Birkholz (1), Lucia Malone (1), Peter C. Fineran (1,2) (1) Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand, (2) Bio-Protection Research Centre, University of Otago, PO Box 56, Dunedin 9054, New Zealand |
[P.056] Programmable RNA Recognition and Cleavage by N. meningitidis CRISPR-Cas9 Beth A. Rousseau†, Zhonggang Hou†, Max J. Gramelspacher, Yan Zhang* Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA; † These authors contributed equally |
| [P.057] The role of Cas4 in CRISPR adaptation Kieper S.N.* (1), Almendros C. (1), Haagsma A. (1), McKenzie R.E., Niessen M. (1), Brouns S.J.J. (1,2) (1) Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, Netherlands, (2) Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, Netherlands |
[P.058] Regulators of CRISPR-Cas adaptive immune systems in Serratia Mariya S. Yevstigneyeva*, Hannah G. Hampton, Peter C. Fineran Department of Microbiology and Immunology, University of Otago, New Zealand |
| [P.059] Quantitative single-molecule microscopy: Concepts and applications in Microbiology Bartosz Turkowyd, David Virant, Ilijana Vojnovic, Alexander Balinovic, Marc Endesfelder, Haruko Gladys Gölz Carnero, Borbála Pisak-Lukáts, Jannik Winkelmeier, Ulrike Endesfelder* Max Planck Institute for Terrestrial Microbiology, Marburg; LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany |
[P.060] The mechanism of DNA cleavage by Cas12a Benedict G. Tan, Kara van Aelst, Mark Szczelkun* DNA-Protein Interactions Unit, School of Biochemistry, University of Bristol, Bristol, UK |
| [P.061] Ongoing CRISPR interference leads to bimodal distribution of plasmid copy number in cells Alexander Martynov* (1), Viktor Mamontov (1), Ekaterina Semenova (2), Jaroslav Ispolatov (1,3), Konstantin Severinov (1,2) (1) Skolkovo Institute of Science and Technology, Moscow, Russia, (2) Rutgers University, New Brunswick, NJ, USA (3) Universidad de Santiago de Chile, Santiago, Chile |
[P.062] The impact of biodiversity on phage immunity and virulence in Pseudomonas aeruginosa Ellinor O Alseth*, Ellie Pursey, Angus Buckling, Edze R Westra ESI and CLES, University of Exeter, UK |
| [P.063] The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR–Cas subtype III-Bv system Juliane Behler* (1), Kundan Sharma (2), Viktoria Reimann (1), Annegret Wilde (3), Henning Urlaub (2), Wolfgang R. Hess (1) (1) Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Freiburg, Germany, (2) Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany, (3) Molecular Genetics, Faculty of Biology, University of Freiburg, Freiburg, Germany |
[P.064] Cellular Indel Profiles and Dynamics induced by different CRISPR/Cas9 Delivery Formats: Applying in vitro Methodologies ex vivo Eric Paul Bennett* University of Copenhagen, Copenhagen Center for Glycomics, Department of Odontology, Maersk Tower 7.10.32, Noerre Alle 14, Copenhagen N., Denmark |
| [P.065] Crystal structure of Cas10/Csm4 subcomplex bound with ATP Kwang-Hyun Park* (1), In-Young Baek (1), Eui-Jeon Woo* (1,2) (1) Disease Target Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, South Korea, (2) Department of Analytical Bioscience, University of Science and Technology, Daejeon 305-333, South Korea |
[P.066] Nus factors prevent transcription termination of CRISPR arrays in diverse bacterial species Anne Stringer (1), Gabriele Baniulyte (2), Joseph Wade* (1,2) (1) Wadsworth Center, New York State Department of Health, Albany, NY, USA, (2) Department of Biomedical Sciences, University at Albany, Albany, NY, USA |
| [P.067] The Kinetics of Plasmid Loss in the Type I-E CRISPR-Cas System Rebecca E. McKenzie* (1,2), Jasper van Lopik (1), Sander J. Tans (1,2), Stan J. J. Brouns (2,3) (1) Department of Bionanoscience, Delft University of Technology, 2629 HZ, Delft, the Netherlands, (2) AMOLF, Science Park 104, 1098 XG Amsterdam, the Netherlands, (3) Laboratory of Microbiology, Wageningen University, 6708 WE Wageningen, the Netherlands |
[P.068] Structural Basis of Type IV CRISPR RNA Biogenesis Ryan Jackson*, Emily Warner, Hannah Taylor, Valerie Crowley Department of Chemistry and Biochemistry, Utah State University, USU |
| [P.069] Interplay of Host Nucleases, RecBCD and CRISPR-Cas Adaptation in Escherichia coli Marin Radovčić (1), Lukas Wettstein (2), Tom Killelea (2), Edward L. Bolt (2), Ivana Ivančić-Baće* (1) (1) Faculty of Science, Division of Molecular Biology, Department of Biology, University of Zagreb, Croatia, (2) School of Life Sciences, University of Nottingham, United Kingdom |
[P.070] Sequence Functionality of DNA Binding Motifs in Nuclear Hormone Signaling Edwin Cheung*, Weng Sam Chan, Guimei Cui Faculty of Health Sciences, University of Macau, Taipa, Macau |
| [P.071] PAMs Effect on Cascade Recognition and Cas3 Recruitment in a Type I-B CRISPR-Cas System John Mallon*, Brian Krahl, Scott Bailey Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA |
[P.072] Crystal Structure of Cas9 from B. laterosporus Toshihiro Nakane* (1), Seiichi Hirano (1), Takashi Yamano (1), Takanori Nakane (2), Ryuichiro Ishitani (1), Hiroshi Nishimasu (1), Osamu Nureki (1) (1) Department of Biological Science, Graduate School of Science, The University of Tokyo, Japan, (2) Cambridge Biomedical Campus, UK |
| [P.073] Preference for specific spacer donor molecules by the CRISPR-Cas I-E system of E. coli Noemí M. Guzmán* (1), Enrique Viguera (2), Rafael Maldonado (1), Jesús García-Martínez (1), Francisco J.M. Mojica (1,3) (1) Departamento de Fisiología, Genética y Microbiología. Universidad de Alicante, Campus de San Vicente, 03690 San Vicente del Raspeig (Alicante), Spain, (2) Área de Genética. Facultad de Ciencias. Universidad de Málaga,Campus de Teatinos 29071 Málaga, Spain, (3) I.M.E.M. Ramón Margalef. Universidad de Alicante, Campus de San Vicente, 03690 San Vicente del Raspeig (Alicante), Spain |
[P.074] DNA unwinding is the primary determinant of CRISPR-Cas9 activity Shanzhong Gong (1), Helen H. Yu (1), Kenneth A. Johnson (1), David W. Taylor* (1,2) (1) Department of Molecular Biosciences, University of Texas at Austin, USA, (2) Center for Systems and Synthetic Biology, University of Texas at Austin, USA |
| [P.075] Integration Host Factor influences CRISPR-Cas immunity Robert D. Fagerlund*, Tim J. Ferguson, Bridget N.J. Watson, Peter C. Fineran Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand |
[P.076] Hybridization Kinetics Explains CRISPR-Cas Off-Targeting Rules Misha Klein* (1), Behrouz Eslami-Mossallam (1), Dylan Gonzalez Arroyo (1,2), Martin Depken (1) (1) Kavli Institute of NanoScience and Department of BioNanoScience, Delft university of Technology, Delft 2629HZ, the Netherlands, (2) Mathematical Institute, Leiden University, 2333CA Leiden, the Netherlands |
| [P.077] Comprehensive search for Type III accessory genes reveals 39 new non-CARF accessory protein families Shiraz A Shah* (1), Omer S. Alkhnbashi (2), Juliane Behler (3), Wenyuan Han(1), Qunxin She (1), Wolfgang R Hess (3), Roger A Garrett (1), Rolf Backofen (2) (1) Danish Archaea Centre, University of Copenhagen, Denmark, (2) Freiburg Bioinformatics Group, University of Freiburg, Germany, (3) Genetics and Experimental Bioinformatics, University of Freiburg, Germany |
[P.078] Evolution and diversification of CRISPR-Cas systems in microbial communities Dina Berenbaum* (1), Dor Russ (2), Roy Kishony (2,3) (1) Technion, Grand Energy program, Technion City, Haifa 32000, Israel, (2) Technion, Faculty of Biology, Technion City, Haifa 32000, Israel, (3) Technion, Faculty of Computer Science, Technion City, Haifa 32000, Israel |
| [P.079] Analysis of the putative ligand binding to CARF domain of Csx1 protein using molecular docking and Molecular Dynamics simulations Visvaldas Kairys*, Česlovas Venclovas Department of Bioinformatics, Institute of Biotechnology, Vilnius University, Lithuania |
[P.080] Unravelling the energy landscape of CRISPR-dCas9 target recognition by physical modelling Behrouz Eslami-Mossallam*, Koen van der Sanden, Misha Klein, Martin Depken Kavli Institute of NanoScience and Department of BioNanoScience, Delft University of Technology, Delft 2629HZ, the Netherlands. |
| [P.081] Organization and dynamics of the Type I-Fv CRISPR-Cas system of Shewanella putrefaciens CN-32 studied at a high spatiotemporal resolution Bartosz Turkowyd* (1,2), Hanna Müller-Esparza (1), Lennart Randau (1), Ulrike Endesfelder (1,2) (1) Max Planck Institute for Terrestrial Microbiology, (2) LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany |
[P.082] On the origin(s) and evolutionary relationships of the reverse transcriptases associated with type III CRISPR-Cas systems A. González-Delgado*, F. Martínez-Abarca*, M.R. Mestre, N. Toro Structure, Dynamics and Function of Rhizobacterial Genomes (Grupo de Ecología Genética de la Rizosfera), Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, 18008, Granada, Spain |
| [P.083] Functions of the CRISPR-associated protein Cas2 in Haloferax volcanii Pascal Dieter Märkle*, Anita Marchfelder Ulm University, Department of Biology II, 89069, Ulm, Germany |
[P.084] guideSim: Metric Learning for evaluation of CRISPR effector efficiency Bogdan Kirillov* (1), Ekaterina Savitskaya (2) (1) Center of Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Russian Federation, (2) Institute of Molecular Genetics, Russian Academy of Sciences, Russian Federation |
| [P.085] Gp5.9 of Bacteriophage T7 inhibits CRISPR adaptation in Type I-E CRISPR-Cas system of Escherichia coli Elena Fomenko* (1), Konstantin Severinov† (1,2,3,4), Udi Qimron† (5) (1) Skolkovo Institute of Science and Technology, Moscow, Russi, (2) Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia, (3) Waksman Institute of Microbiology, Piscataway, NJ, USA, (4) Peter the Great St. Petersburg State Polytechnic University, St. Petersburg, Russia, (5) Sackler School of Medicine, Tel Aviv University, Israel; † Equal supervision |
[P.086] Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption Ramu Gopalappa* (1), Bharathi Suresh (2), Suresh Ramakrishna (3,4), Hyongbum (Henry) Kim (1,2) (1) Department of Pharmacology, Yonsei University College of Medicine, Yonsei University, Seoul 03722, South Korea, (2) Brain Korea 21 Plus Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, South Korea, (3) Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, South Korea, (4) College of Medicine, Hanyang University, Seoul 04763, South Korea |
| [P.087] Escape of RNA phage from RNase III Murat Buyukyoruk*, Blake Wiedenheft* Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA |
[P.088] Increasing of cell resistance to genotoxic stress by multiplex overexpression of DNA damage recognition genes Ilya Velegzhaninov* (1,2), Anna Rybak (1), Elena Belykh (1), Yana Pylina (1), Dmitry Shadrin (1) (1) Institute of Biology of Komi Science Centre of Ural Branch of RAS, Russia, (2) Polytechnic Institute of Vyatka State University, Russia |
| [P.089] High – throughput characterization of PAM sequences in Escherichia coli Type I-E CRISPR-Cas system Olga Musharova* (1,3), Ekaterina Savitskaya (1,2), Vasily Sitnik (1), Konstantin Severinov (1,2) (1) Institute of Science and Technology, Skolkovo, Russia, (2) Institutes of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia |
[P.090] Synthetic sgRNA Enables Highly Efficient CRISPR Editing of Primary Cells for Therapeutic Applications John Walker* (1), Kevin Holden (1), Yuxuan Wu (2), Michelle Christian (3), Mara Pavel-Dinu (4), Daniel Dever (4) Volker Wiebking (4), Rasmus Bak (5), Dane Hazelbaker (6), Imran House (7) (1) Synthego, Redwood city, CA, USA, (2) Boston Children’s Hospital, Boston, MA, USA, (3) Seattle Children’s Hospital, Seattle, WA, USA, (4) Stanford University, Stanford, USA, (5) Aarhus University, Aarhus, Denmark, (6) Broad Institute of MIT, Cambridge, MA, USA, (7) Peter MacCallum Cancer Centre, Parkville,Victoria, Australia |
| [P.091] Activation of CRISPR associated ribonucleases by oligoadenylates Januka S. Athukoralage*, Christophe Rouillon, Shirley Graham, Malcolm F. White Biomedical Sciences Research Complex, University of St Andrews, United Kingdom |
[P.092] Harnessing RNA targeting CRISPR systems for transcriptome engineering and human health Gootenberg, J.S.*† (1,2,3,4,7), Abudayyeh, O.O.† (1,2,3,4,5), Cox, D.B.T (1,2,3,4,5,6), Franklin, B. (1,2,3,4), Kellner, M. (1,2,3,4), Han, S. (8), Joung, J. (1,2,3,4), Belanto, J. (9), Voytas, D.F. (9), Ting, A.Y. (8), and Zhang, F. (1,2,3,4) (1) Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA, (2) McGovern Institute for Brain Research at MIT, (3) Department of Brain and Cognitive Science, (4) Department of Biological Engineering, (5) Department of Health Sciences and Technology, (6) Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, (7)Department of Systems Biology, Harvard University, Boston, MA 02115, USA, (8) Departments of Genetics, Biology, and Chemistry, Stanford University, Stanford, CA 94305, USA, (9) Department of Genetics, Cell Biology &Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA; † These authors contributed equally |
| [P.093] Interaction of bacterial resistance strategies and phage lifestyle choice Clare Rollie*, Edze Westra Environment and Sustainability Institute, University of Exeter, United Kingdom |
[P.094] RNA-guided RNA-targeting type VI-A CRISPR-Cas system: spacer acquisition and phage response to cell resistance Ekaterina Semenova* (1), Natalia Rubanova (2), Vasily Sitnik (2), Aleksandra Kim (1), Ishita Jain (1), Kirill A. Datsenko (3), Konstantin Severinov (1,2) (1) Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, USA, (2) Skolkovo Institute of Science and Technology, Skolkovo, Russia, (3) Department of Biological Sciences, Purdue University, West Lafayette, IN, USA |
| [P.095] Control of cyclic oligoadenylate synthesis in a type III CRISPR system Christophe Rouillon*, Januka S Athukoralage, Shirley Graham, Malcolm F White Biomedical Sciences Research Complex, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK |
[P.096] One-step assembly of CRISPR arrays advance multiplexing applications and reveal design constraints Chunyu Liao* (1,2), Fani Ttofali (1), Rebecca Slotkowski (1), Steven Denny (1), Taylor D. Cecil (1), Ryan Leenay (1), Albert J. Keung (1), Chase L. Beisel (1,2) (1) Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, US, (2) Helmholtz Institute for RNA-based Infection Research, Würzburg, Germany |
| [P.097] Target recognition dynamics of the CRISPR-Cas surveillance complex Cascade Pierre Aldag* (1), Marius Rutkauskas* (1), Julene Madariaga (2), Inga Songailiene (3), Tomas Sinkunas (3), Virginijus Siksnys (3), Ralf Seidel (1) (1) Debye Institute for Soft Matter Physics, Leipzig University, Germany, (2) Centro Nacional de Biotecnología (CSIC), Madrid, Spain, (3) Institute of Biotechnology, Vilnius University, Lithuania |
[P.098] CRISPR/Cas9 screening using unique molecular identifiers Jenna Persson* (1,2), Sandeep K. Botla (2), Bernhard Schmierer (1,2) (1) High Throughput Genome Engineering, Science for Life Laboratories, Stockholm, Sweden, (2) Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden |
| [P.099] Mechanistic insights into the activity of anti-CRISPR proteins from virulent streptococcal phages Adeline Goulet (1), Beatrice Amiges (1), Renaud Vincentelli (1), Olivier Fuchsbauer (1), Claire Zimberger (1), Alain Roussel (1), Alexander Hynes (2), Sylvain Moineau (2), Christian Cambillau* (1) (1) Architecture et Fonction des Macromolécules Biologiques, CNRS & Aix-Marseille Université, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France, (2) Département de biochimie, de microbiologie, et de bioinformatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Université Laval, Québec City, QC G1V 0A6, Canada. |
[P.100] Active displacement of bound Cas9 Colin Maxwell (1), Ryan Marshall (2), Fani Ttofali (1), Mary Dunlop (3), Vincent Noireaux (2), Chase Beisel* (1,4) (1) North Carolina State University, USA, (2) University of Minnesota, USA, (3) Boston University, USA, (4) Helmholtz Institute for RNA-based Infection Research, Germany |
| [P.101] Adaptation singularities of CRISPR-Cas I-E variants in E. coli Noemí M. Guzmán (1), Cristóbal Almendros (2), Rafael Maldonado (1), Jesús García-Martínez (1), Francisco J.M. Mojica* (1,3) (1) Departamento de Fisiología, Genética y Microbiología. Universidad de Alicante, Campus de San Vicente, 03690 San Vicente del Raspeig (Alicante), Spain, (2) Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, Netherlands, (3) I.M.E.M. Ramón Margalef. Universidad de Alicante, Campus de San Vicente, 03690 San Vicente del Raspeig (Alicante), Spain |
[P.102] In search of suitable MTS-UTR pairs for effective RNA-guided endonuclease import into the mitochondrion Nikita Shebanov*, Natalya Verechshagina, Natalya Nikitchina, Ilia Mazunin Laboratory of Molecular Genetics Technologies, Immanuel Kant Baltic Federal University, Russia |
| [P.103] Functions of the CRISPR-associated protein Cas1 in Haloferax volcanii Julia Wörtz*, Anita Marchfelder Ulm University, Department of Biology II, 89069, Ulm, Germany |
[P.104] Bottom-up modelling for CRISPR/(d)Cas9 target prediction Behrouz Eslami-Mossallam† (1), Misha Klein† (1), Koen van der Sanden (1), Dylan Gonzalez Arroyo (1,2), Martin Depken* (1) (1) Kavli Institute of NanoScience and Department of BioNanoScience, Delft university of Technology, Delft 2629HZ, the Netherlands, (2) Mathematical Institute, Leiden University, 2333CA Leiden, the Netherlands; † These authors contributed equally |
| [P.105] Compiling Multicopy Single-Stranded DNA Sequences from Bacterial Genomes Wonseok Yoo, Dongbin Lim*, Sangsoo Kim Department of Bioinformatics and Life Science, Soongsil University, Korea |
[P.106] A new quorum-sensing regulator inhibits CRISPR-Cas expression and adaptive immunity Ping Lin (1,2), Xin-xiang Jiang & Min Wu* (1) (1) Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota 58203-9037, USA, (2) State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, The Third Military Medical University, Chongqing 400042, P. R. China |
| [P.107] Characterization of a Type IV CRISPR ribonucleoprotein complex Ahsen Özcan* (1), Marcus Ziemann (1), Henning Urlaub (2), Thomas Heimerl (3), Lennart Randau (1) (1) Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, Marburg 35043, Germany, (2) Max Planck Institute for Biophysical Chemistry, Am Fassberg 11 , Göttingen, 37077,Germany, (3) LOEWE Research Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany |
[P.108] Issues to consider for making public investment decisions on genome editing Min-Kyu Han*, Byoung Min Ahn Korea Institute of S&T Evaluation and Planning (KISTEP), South Korea |
| [P.109] Ribonuclease activity of Csm6 is required for anti-plasmid immunity by Type III-A CRISPR-Cas systems Kawanda Foster*, Joshua Kalter, Walter Woodside, Rebecca M. Terns, Michael P. Terns University of Georgia, USA |
[P.110] Identification and classification of CRISPR-Cas Systems among the Asgard Archaea Tanner R Wiegand* (1), Kiley W Seitz (2), Brett J Baker (2), Blake Wiedenheft* (1) (1) Department of Microbiology and Immunology, Montana State University, USA, (2) Department of Marine Science, University of Texas-Austin, USA |
| [P.111] Conformational control of Cas3 recruitment by a crRNA-guided surveillance complex MaryClare F. Rollins* (1), Saikat Chowdhury (2), Joshua Carter (1), Sarah M. Golden (1), Heini M. Miettinen (1), C. Martin Lawrence (3), Gabriel C. Lander (2), Blake Wiedenheft (1) (1) Department of Microbiology and Immunology, Montana State University, USA, (2) Department of Integrative Structural and Computational Biology, Scripps Research Institute, USA, (3) Department of Chemistry and Biochemistry, Montana State University, USA |
[P.112] Natural diversity of CRIPSR spacers in thermophiles Sofia Medvedeva* (1,2,4), Ying Liu (2), Anna Lopatina (3), David Prangishvili (2), Mart Krupovic (1,2), Konstantin Severinov (4) (1) Sorbonne Universités, UPMC Univ, France, (2) Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Department of Microbiology, Institut Pasteur, France, (3) Department of Molecular Genetics, Weizmann Institute of Science, Israel, (4) Center for Data-Intensive Biomedicine and Biotechnology, Skoltech, Russia |
| [P.113] BREX system of E. coli distinguishes self from non-self by methylation of a specific DNA site Julia Gordeeva* (1), Natalya Morozova (1,2), Nicolas Sierro (3), Artem Isaev (1), Ksenia Tsvetkova (1), Mikhail Matlashov (1), Nikolai Ivanov (3), Lanying Zeng (4) and Konstantin Severinov (1,2,5) (1) Skolkovo Institute of Science and Technology, Moscow, Russia, (2) Peter the Great St. Petersburg State Polytechnic University, St. Petersburg, Russia, (3) Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland, (4) Department of Biochemistry and Biophysics, Center for Phage Technology, Texas A&M University, College Station, TX 77843, USA, (5) Waksman Institute of Microbiology, Piscataway, New Jersey, USA |
[P.114] Impact of CRISPR-Cas on Plasmid Stability Rafael Pinilla-Redondo*, Jonas Stenløkke Madsen, Gisle Vestegaard, Joseph Nesme, Henriette Lyn Røder, Søren Johannes Sørensen Section of Microbiology, University of Copenhagen, Denmark |