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Program

Please note this is a preliminary programme, which could undergo minor changes.

June 20, Wednesday

07:45-08:45 Registration
08:45-09:00 Welcome address
09:00-10:00 Keynote talk:
Feng Zhang, MIT
Advances in genome editing technologies
10:00-10:30 Eugene Koonin, NIH/NCBI
Origin and evolution of CRISPR-Cas systems
10:30-11:10 Coffee break
Class 1 systems
Chair: John van der Oost
11:10-11:40 Blake Wiedenheft, Montana State University
dsDNA unwinding is a necessary signal for Cas3 activation
11:40-12:00 Ralf Seidel, Universität Leipzig
A kinetic model for the off-target recognition by Cascade
12:00-12:20 Joachim Vink, TU Delft
Direct visualization of CRISPR target search in live bacteria
12:20-12:40 Rodolphe Barrangou, NC State University
Characterization and applications of Type I CRISPR-Cas systems in bacteria and beyond
12:40-13:00 Paul Donohoue, Caribou Biosciences, Inc.
Enabling Genome Engineering in Human Cells with the Type I CRISPR-Cas System
13:00-14:20 Lunch
Chair: Erik Sontheimer
14:20-14:50 Martin Jinek, University of Zurich
Structural and mechanistic insights into type III-A and type V-A systems
14:50-15:10 Tina Y. Liu, University of California, Berkeley
Recruitment of the Type III-A Csm complex to transcriptionally active DNA by RNA-guided RNA recognition
15:10-15:30 Yanli Wang, Institute of Biophysics, Chinese Academy of Sciences
Structural studies of Type III-A CRISPR-Cas system
15:30-15:50 Nora Pyenson, Rockefeller University
Broad Targeting Specificity during Type III Immunity Constrains Viral Escape
15:50-16:30 Coffee break
Chair: Dipali Sashital
16:30-17:00 Kira S. Makarova, NIH/NCBI
Beyond the adaptive immunity: sub- and neofunctionalization of CRISPR-Cas systems and their components
17:00-17:30 Peter Fineran, University of Otago
CRISPR-Cas-mediated phage resistance enhances horizontal gene transfer by transduction
17:30-17:50 Lennart Randau, Max Planck Institute for Terrestrial Microbiology
The Type IV crRNP surveillance complex of Aromatoleum aromaticum
Poster session I
17:50-19:50 Poster session (odd numbers)
20:00
20:20
Conference dinner
Promoting diversity discussion panel with Rachel Haurwitz, Karen Maxwell, and Dipali Sashital

June 21, Thursday

Class 2 systems
Chair: Michael Terns
09:00-09:30 John van der Oost, Wageningen University
DNA targeting by CRISPR Class 2 nucleases
09:30-09:50 Daan Swarts, University of Zurich
Structural insights into the mechanism of DNA cleavage by CRISPR-Cas12a/Cpf1
09:50-10:10 Ilya Finkelstein, University of Texas at Austin
A molecular basis for the extraordinary specificity of Cas12a (Cpf1)
10:10-10:30 David Scott, Arbor Biotechnologies
Discovery of Type VI-D CRISPR-Cas Systems
10:30-10:50 Guilhem Faure, NIH/NLM/NCBI
ncRNAs in Class 2 CRISPR-Cas systems: CRISPR array as an antirepeat generator
10:50-11:30 Coffee break
Chair: Blake Wiedenheft
11:30-12:00 Osamu Nureki, University of Tokyo
Molecular mechanism of CRISPR and structure-based development of genome editing tool towards medical applications
12:00-12:30 Scott Bailey, Johns Hopkins University
Real-time observation of DNA target interrogation and cleavage by engineered Cas9s and Cas12a
12:30-12:50 Hiroshi Nishimasu, University of Tokyo
Engineered CRISPR-Cas9 recognizing a single guanine PAM
12:50-13:10 Virginijus Siksnys, Vilnius University
Exploring Cas9 diversity
13:10-14:30 Lunch
Chair: Karen Maxwell
Genome editing with Class 2 systems
14:30-15:00 Rachel Haurwitz, Caribou Biosciences
Enabling Precision Editing with CRISPR Hybrid RNA-DNA Guided Cas9
15:00-15:30 Erik Sontheimer, University of Massachusetts Medical School
Enhancing genome editing by chemical modification of guides and donors
15:30-15:50 Mollie Schubert, Integrated DNA Technologies
A novel Cas9 mutant confers reduced off-target gene editing while maintaining on-target potency
15:50-16:30 Coffee break
Chair: Rotem Sorek
16:30-17:00 Barrett Steinberg, Editas
Identification of guide-intrinsic determinants of Cas9
17:00-17:20 Akihiko Kondo, Kobe University
Genome editing with base editing systems from bacteria to plants
17:20-17:40 Ian Jepson, Syngenta
New crop breeding approaches using CRISPR Cas9 editing
17:40-18:00 Mark Cigan, Genus plc
Gene editing to tackle diseases in livestock
Poster session II
18:00-20:00 Poster session (even numbers)
20:00 Free evening in Vilnius

June 22, Friday

Anti-CRISPR
08:40-09:10 Karen L. Maxwell, University of Toronto
Inhibition of Type II-C CRISPR-Cas Systems by anti-CRISPR Proteins
09:10-09:40 Sylvain Moineau, Laval University
Type II-A anti-CRISPRs are widespread in virulent phages infecting Streptococcus thermophilus
09:40-10:00 Adeline Goulet, CNRS
Mechanistic insights into the activity of anti-CRISPR proteins from virulent streptococcal phages
10:00-10:20 Adair Borges, UCSF
Bacteriophage cooperation suppresses CRISPR-Cas3 and Cas9 immunity
10:20-11:00 Coffee break
CRISPR technology – gene regulation
Chair: Rachel Haurwitz
11:00-11:20 Patrick Hsu, Salk Institute for Biological Studies
Transcriptome engineering with RNA-targeting type VI-D CRISPR effectors
11:20-11:40 Emily Anderson, Horizon Discovery
Potent transcriptional activation using CRISPRa and synthetic crRNA:tracrRNA
11:40-12:00 David Bikard, Institut Pasteur
Silencing genes with dCas9: a story of fine-tuned control, high-throughput screens and surprising toxicity
CRISPR technology – diagnostics
12:00-12:20 Omar Abudayyeh, Broad Institute/Harvard/MIT
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
12:20-12:40 Janice Chen, UC Berkeley
DETECTR: how Cas12a (Cpf1) mysteries led to a DNA detection platform
12:40-14:00 Lunch
Adaptation
Chair: Sylvain Moineau
14:00-14:30 Ailong Ke, Cornell University
Spacer acquisition mechanism in type II-A CRISPR system
14:30-14:55 Dipali Sashital, Iowa State University
Cas4-dependent prespacer processing ensures high-fidelity programming of CRISPR arrays
14:55-15:20 Michael Terns, University of Georgia
Cas4 nucleases define the PAM, length, and orientation of DNA fragments integrated at CRISPR loci
15:20-15:45 Stan Brouns, Delft University of Technology
On the role of Cas4 in CRISPR adaptation
15:45-16:25 Coffee break
Chair: Malcolm White
16:25-16:45 Ville Hoikkala, University of Jyvaskyla
Phage-induced spacer acquisition in type VI-B and II-C CRISPR loci of the native host Flavobacterium columnare
16:45-17:15 Konstantin Severinov, Rutgers University
Detection of priming adaptation intermediates in vitro
Beyond CRISPR
17:15-17:45 Rotem Sorek, Weizmann Institute of Science
New defense systems protecting bacteria from phage infection
17:45-18:00 Concluding remarks
18:30
19:00
Conference Gala dinner
Meet the editors discussion panel with April Pawluk (Cell), John Pham (Mol Cell), Steve Mao (Science), Angela Eggleston (Nature), Claudio Nunes Alves (Nat Micro), and Kevin Davies (CRISPRj)

June 23, Saturday

9:15-16:00 Conference tour to Trakai (optional, by reservation only)
The buses will depart (9:15) and return (16:00) to the conference venue.
The tour will include outdoor activities, so please make sure to have suitable footwear and protection against the unpredictable rain.